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. 2016 Jan 29;11(1):e0147764. doi: 10.1371/journal.pone.0147764

Fig 3. Overexpression of FoxC2 promotes NP cells growth.

Fig 3

(A) Immunohistochemical staining of NP cells against Ki-67. Nuclei were stained with DAPI, shown in blue. Images were acquired using laser scanning confocal microscopy under a 40×objective, and had Ki-67-positive percentages in cultured NP cells 48h after transfection with LentiFoxC2 or LentiGFP or no transfection. (B) Real-time RT-PCR analysis of Ki-67 mRNA expression in NP cells after transfection of LentiFoxC2 for 0h, 24h, 48h or 72h. (C) Growth of NP cells was shown after transfection with 50 nmol/L of LentiFoxC2 or LentiGFP or no transfection. The growth index was assessed at 1, 2, 3, 4, and 5 days. Error bars represent SD. As compared with control, * indicates p<0.05, ** indicates p<0.01.