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. 2016 Jan 29;11(1):e0147764. doi: 10.1371/journal.pone.0147764

Fig 5. The interactional promotion effect between FoxC2 and BMP-7 signaling pathway.

Fig 5

(A) FoxC2 mRNA expression levels were examined by real-time PCR in the presence and absence of SiRNA-Smad1 the BMP inhibitor noggin and Scramble. (B) NP cells were treated 100 ng/ml BMP7, 100 ng/ml noggin for 2h and the total RNA was extracted to perform real-time PCR of FoxC2 genes (C) Smad1/5/8 phosphorylation Protein expression in NP cells was transfected with 50 nmol/L of Lenti FoxC2 or Lenti GFP or no transfection. (D) Expression levels of BMP-7 were examined by real-time PCR after transfection of 50 nmol/L of Lenti FoxC2 or Lenti GFP or no transfection. Error bars represent SD. As compared with control, * indicates p<0.05, ** indicates p<0.01.