Skip to main content
. 2016 Jan 29;11(1):e0147764. doi: 10.1371/journal.pone.0147764

Fig 6. FoxC2 enhances the compensatory anabolic gene expression effect by BMP-7.

Fig 6

NP cells were treated 100 ng/ml BMP7, 100 ng/ml noggin or 50 nmol/L of LentiFoxC2 for 2h. After stimulation, cells were harvested and the total RNA was extracted to perform real-time PCR of (A) collagen type-II genes and (B) aggrecan. (C) NP cells were stimulated by 50 nmol/L of Lenti FoxC2, BMP-7 (100 ng/ml), and cocktail of Lenti FoxC2 and BMP-7 for 2h. After stimulation, cells were harvested and the total RNA of Noggin was extracted to perform real-time PCR. As compared with control, ***p<0.01, *p<0.05, and **p<0.01. (D) Cell lysates were then prepared and analyzed by western blotting with specific anti-Noggin antibody. β-actin was used as the control for normalization. Error bars represent SD. As compared with control, * indicates p<0.05, ** indicates p<0.01.