Table 2. Primer Sequences for PCR.
Primers | Forward | Reverse | Tm (°C) |
---|---|---|---|
FoxC2 | 5’-CGCCTAAGGACCTGGTGAAG-3’ | 5’-GGAAGCGGTCCATGATGA-3 | 56 |
BMP-7 | 5’-GGTGGCAGGACTGGATCATC-3’ | 5’-GCGCATTCTCCTTCACAGTAATAC-3’ | 56 |
Aggrecan | 5’-AAGGACTGTCTATCTGCACGCCAA-3’ | 5’-TCACCACCCACTCCGAAGAAGTTT-3’ | 56 |
Collagen Type II | 5’-TCATAAGGATGTGTGGAAGCCCGA-3’ | 5’-GGCTGAGGCAGTCTTTCATGTCTT-3’ | 56 |
Noggin | 5’-AGCGAGATCAAAGCGCTGGAGTT-3’ | 5’-TCTGTAACTTCCTCCGCAGCTTCT-3’ | 56 |
Ki-67 | 5’-TCCTTTGGTGGGCACCTAAGACCTG-3 | 5’-TGATGGTTGAGGTCGTTCCTTGATG-3 | 56 |