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. 2016 Jan 29;11(1):e0147831. doi: 10.1371/journal.pone.0147831

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© 2016 Daryadel et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Immunoflourescence staining for the (P)RR/Atp6p2 in mouse kidney (green). (A) Overview showing (P)RR/Atp6ap2 (green), the principal cell specific AQP2 water channel (red), and nuclei (blue), original magnification 40 x. (B) A cortical field with glomerulus (G), and (P)RR/Atp6ap2 staining (green) in the proximal tubule (PT) and connecting tubules (CNT), 400 x magnification. (C) Cortical and outer medullary collecting duct stained for (P)RR/Atp6ap2 (green) and the principal cell specific AQP2 water channel (red), 400 x magnification. (E,D,F) (P)RR staining (D: green) was detected in the proximal tubule (PT) in the brush border membrane and colocalized with the a4 H⁺-ATPase subunit (ATP6V0A4)(E: red) as indicated by the yellow color (F), original magnification 400x.