Fig 1. The seed phenotype of two parent lines and two extreme pools were selected by 1000-seed weight (TSW) data for SLAF-sequencing.

(A) Seeds of the large-seed line G-42 and the small-seed line 7–9. (Scale bar, 1 mm) (B) Forty-six lines with the heaviest TSW and 47 lines with the lightest TSW were selected and pooled for SLAF-sequencing (the histogram was drawn based on the TSW data collected from 250 DH lines in May, 2014).