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. 2016 Jan 29;11(1):e0148137. doi: 10.1371/journal.pone.0148137

Fig 3. S1P1 is involved in S1P-driven chemotactic responses of T-ALL blasts.

Fig 3

T-ALL blasts were serum-starved for 2 h and pre-treated or not with W146 (100 μM). (A) Cells were applied to Transwell™ chambers with S1P 1, 10, 100, 500 or 1000 nM and incubated for 4 hours (n = 3). (B) Migratory response of CEM cells toward S1P 100, 2500, 5000 and 10000 nM (n = 3). Values correspond to a specific migration after subtracting the numbers of migrating cells obtained in wells with culture medium only. Black bars correspond to T-ALL blasts pre-treated with RPMI-BSA 0.1% and white bars correspond to T-ALL blasts pre-treated with W146. Results are expressed as mean ± SEM and were analyzed by unpaired Student’s t test. Differences were considered statistically significant when * p˂0.05, ** p ˂0.01 or *** p ˂0.001.