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(A, E) PBS, (B, F) ClearT2, (C, G) SeeDB, and (D, H) CUBIC. ZsGreen1 expression in the liver was detected by CLSM. (A–D) Depth coding. The color chart indicates depth on the Z-axis. (E–H) Maximum intensity projection (X–Z plane). Scale bar: 100 μm. Acquisition conditions: lens, 20× dry; laser, 488 nm; output, 5%; emission, 492–540 nm; master gain, (A, E) 432–1200, (B, F) 433–1129, (C, G) 405–1129, and (D, H) 492–1115.
