Membrane fusion between R18–PM and ROS disk membranes. A representative tracing of the change in fluorescence observed with the R18 lipid mixing assay, at 37°, is shown. (a) Fusion is initiated with the simultaneous addition of R18–PM and calcium ([Ca2+]free = 45 mM) to the disk membranes, (b) The addition of Triton X-100. [Reprinted with permission from K. Boesze-Battaglia, A. D. Albert, and P. L. Yeagle, Biochemistry
31, 3733 (1992); American Chemical Society.]