Table I. Comparison of R18-Labeled Plasma Membrane Fusion with Various Target Membranesa.
Target membrane | Addition | Lag time (sec) | IRF (%) = ΔF/time |
---|---|---|---|
Disks | 10 μM Ca2+ | <20 | 2.9 ± 0.04 |
Rim-specific vesicles | 10 μM Ca2+ | 20–50 | 0.26 ± 0.02 |
Peripherin/rds LUVs | 10 μM Ca2+ | 120 | 0.30 ± 0.03 |
Fusion of R18–PMs with disk membranes,12 isolated rim-specific vesicles, and peripherin/rds recombinant LUVs23 at 37°. The initial rate of fusion is given as the percent change in fluorescence intensity per unit time (minutes with disk membranes and seconds with rim-specific vesicles and peripherin/rds LUVs).