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. Author manuscript; available in PMC: 2016 Jan 29.
Published in final edited form as: Methods Enzymol. 2000;316:65–86. doi: 10.1016/s0076-6879(00)16717-5

Table I. Comparison of R18-Labeled Plasma Membrane Fusion with Various Target Membranesa.

Target membrane Addition Lag time (sec) IRF (%) = ΔF/time
Disks 10 μM Ca2+ <20 2.9 ± 0.04
Rim-specific vesicles 10 μM Ca2+ 20–50 0.26 ± 0.02
Peripherin/rds LUVs 10 μM Ca2+ 120 0.30 ± 0.03
a

Fusion of R18–PMs with disk membranes,12 isolated rim-specific vesicles, and peripherin/rds recombinant LUVs23 at 37°. The initial rate of fusion is given as the percent change in fluorescence intensity per unit time (minutes with disk membranes and seconds with rim-specific vesicles and peripherin/rds LUVs).