Table 2.
Protein sequence coverage of the MDDC proteins commonly identified by two sample preparation workflows. MDDC proteins were precipitated by acetone with wash (pAwA) method and the acetone-washed protein pellets were solubilised by 0.5M TEAB dissolution buffer (sT) or 0.5M TEAB with additional 1% Na-DOC (sTD). Proteins were identified with global FDR 1% and associated distinct peptides were identified with local FDR 5%.
| Experimental replicate |
Triton X-100 cell lysate (TCL) | RIPA cell lysate (RCL) | ||||
|---|---|---|---|---|---|---|
| # of proteins | pAwA-sT | pAwA-sTD | # of proteins | pAwA-sT | pAwA-sTD | |
| #1 | 657 | 20.0 | 21.7 | 793 | 20.3 | 20.8 |
| #2 | 725 | 20.7 | 21.6 | 739 | 20.4 | 21.0 |
| #3 | 715 | 20.0 | 21.3 | 751 | 20.3 | 20.4 |
| #4 | 638 | 20.9 | 21.9 | 666 | 21.0 | 22.2 |
| Average | - | 20.4 | 21.6 | - | 20.5 | 21.1 |