Skip to main content
. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2015 Jun 26;8(1):123–138. doi: 10.1002/wnan.1350

Figure 5. Intracellular assembly and function of elastin-like polypeptide microdomains.

Figure 5

A) An amphiphilic ELP (GFP-S48I48) and an ELP monoblock (dsRED-V96) with similar transition temperatures and molecular weight sort into separate microdomains in live cells. B) An ELP fused with the clathrin-light chain (V96-CLC) is soluble at 31 °C but assembles into V96-CLC microdomains above 37 °C. Red = ELP, Green = the angiotensin II receptor (AngIIR) at the cellular membrane. C) V96-CLC microdomains sequester the machinery of clathrin-mediated endocytosis and inhibit the internalization of AngIIR at 37 and 42 °C. The V96-CLC fusion remains soluble at 31 °C and does not affect receptor internalization. (**p<0.0001) Mean ± 95% confidence interval (n=3). Adapted with permission from Wiley–VCH Verlag GmbH & Co 63, 64.