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. 2016 Jan 29;11(1):e0148097. doi: 10.1371/journal.pone.0148097

Fig 5. ARF6 is required for the activation of the ROS sensitive Erk1/2, p38 and Jnk.

Fig 5

(A) Control and ARF6 depleted VSMC were stimulated with Ang II (100 nM) for the indicated times. Phosphorylation and total levels of Erk1/2, p38, and Jnk were examined (n = 3, **P < 0.01). (B) Cells were treated with vehicle or DPI (10 μM) and stimulated with Ang II (100 nM) for the indicated times. Phosphorylation levels of Erk1/2, p38 and Jnk were assessed as in (A). Results are representative of three independent experiments and quantifications are the mean ± SEM (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001). (C) Cells were treated with vehicle, AG1478 (100 nM) or ML171 (5 μM) for 30 min then stimulated with Ang II for the indicated times. Phosphorylation and total levels of Erk1/2, p38, and Jnk were assessed by Western blot analysis (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001).