Table 2. Intron Positions and splice junction sequences in globin genes of Lucina pectinata, Calyptogena soyoae, Barbatia reeveana, Biomphalaria glabrata and Anadara trapezia.
| Globin | Intron | Position | Size (bp) | Splicing donor (A/C)AGgt (a/g) agt | Splicing acceptor (c/t)richn(c/t) agG/A |
|---|---|---|---|---|---|
| Lucina pectinata (this work) | |||||
| HbI | Intron 1a/b (partial) | Pre-coding | ? | ? | ttttccAGC |
| HbI | Intron 2 | B12.2 | 698 | GGCgtgagt | cgacagTCT |
| HbI | Intron 3 | G7.0 | 1517 | CGGgtaggt | ttttagGCT |
| HbII | Intron 1 | Pre-coding | 2128 | CTTgtaagt | ttacagAGA |
| HbII | Intron 2 | B12.2 | 2666 | GGAgtaaga | acacagTCT |
| HbII | Intron 3 | G7.0 | 1261 | CGTgtatgt | cttcagACC |
| HbIII | Intron 1 | Pre-coding | 1170 | GCAgtgagt | ttatagAAA |
| HbIII | Intron 2 | B12.2 | 933 | GGAgtaaga | ttgcagCCT |
| HbIII | Intron 3 | G7.0 | 1298 | CGAgtatgc | ctgcagGAT |
| Calyptogena soyoae [54] | |||||
| HbII | Intron 1 | A1.3 | 594 | AAGgtaaaa | tttcagCCG |
| HbII | Intron 2 | B12.2 | 312 | AACgtatgt | ttgcagCCT |
| HbII | Intron 3 | G7.0 | 250 | GCGgtaagt | ttttagATT |
| HbI | Intron 1 | A1.3 | 329 | CCAgtaagt | ttttagATG |
| HbI | Intron 2 | B12.2 | 672 | CAAgtaatt | ttttagGCT |
| HbI | Intron 3 | G7.0 | 382 | GCGgtaggt | tttcagATT |
| Barbatia reeveana [50] | |||||
| 2-domain globin (first domain) | Intron 1 | Pre-coding | 3115 | AACgtaagt | ttctagAAT |
| 2-domain globin (first domain) | Intron 2 | B12.2 | 1494 | TTTgtaagt | tcatagATT |
| 2-domain globin (first domain) | Intron 3 | G7.0 | 2175 | GGTgtaagc | ttttagTGG |
| Biomphalaria glabrata [52] | |||||
| Myoglobin | Intron 1 | A3.2 | 1116 | TGCgtaagt | taacagTGA |
| Myoglobin | Intron 2 | B12.2 | 581 | GTGgtgagt | caaaagGAT |
| Myoglobin | Intron 3 | G7.0 | 1008 | GGAgtaagt | tttcagCCT |
| Anadara trapezia [53] | |||||
| Minor Globin | Intron 1 | Pre-coding | ? | ? | ttcagAAT |
| Minor Globin | Intron 2 | B12.2 | 1213 | GAAgtaagt | tcgtagTCT |
| Minor Globin | Intron 3 | G7.0 | 1435 | GGGgtaagt | ttttagAAA |