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. 2016 Jan 29;11(1):e0148089. doi: 10.1371/journal.pone.0148089

Fig 3. AICAR and metformin modify the fatty acid profile in C. elegans.

Fig 3

Individual fatty acids were determined by gas chromatography analysis. Control wild-type (WT) N2 nematodes (white box) were grown in 1 mM AICAR (black box) or in 50 mM metformin (gray box). A) Individual fatty acids: 14:0, myristic acid; 16:0, palmitic acid; 18:0, stearic acid; 16:1n9, 7-palmitoleic acid; 16:1n7, 9-palmitoleic acid; 18:1n9, oleic acid; 18:1n7, vaccenic acid; 18:2n6, linoleic acid; 20:3n6, dihomo-γlinolenic acid (DGLA); 20:4n6, arachidonic acid; 20:4n3, eicosatetraenoic acid; 20:5n3, eicosapentaenoic acid (EPA). B) Fatty acids grouped as saturated (SFA), monounsaturated (MUFA) or polyunsaturated (PUFA) fatty acids. C) Elongase activity indirectly measured from substrate/product ratios. D) Desaturase activity indirectly measured from substrate/product ratios. Each graph shows the mean ± SEM of three independent experiments, *p<0.05, **p<0.01, ***p<0.005 vs. untreated group, one-way ANOVA with Bonferroni's post hoc test using GraphPad Prism.