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. 2016 Jan 29;11(1):e0148089. doi: 10.1371/journal.pone.0148089

Fig 4. AAK activation-dependent changes of selected transcripts involved in energy metabolism regulation in C. elegans.

Fig 4

Gene expression analysis was performed using qRT-PCR on wild-type N2 (WT) and aak-2/ok524 nematodes grown until adults and exposed to 1 mM AICAR for 12 h. A) nhr-49, B) mdt-15, C) sbp-1, D) fat-7, E) fat-2, F) fasn-1, G) acs-2, H) acdh-2 and I) elo-2. The relative expression of each gene was normalized to endogenous 18S rRNA gene expression. Data shown are the mean ± SEM of three independent experiments, *p<0.05, **p<0.01, ***p<0.001 vs. WT control and ##p<0.01, ###p<0.001 vs. WT AICAR, one-way ANOVA with Bonferroni's post hoc test using GraphPad Prism.