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. 2016 Jan 29;11(1):e0148089. doi: 10.1371/journal.pone.0148089

Fig 5. AICAR modifies AAK phosphorylation in mutant strains lacking NHR-49, MDT-15 or SBP-1 transcription factors without altering the expression of both aak-2 and aak-1.

Fig 5

Adult worms of the mutant strains nhr-49/ok2165, mdt-15/tm2182 and sbp-1/ok2363 lacking the proteins NHR-49, MDT-15 and SBP-1, respectively, were exposed to 1 mM AICAR for 12 h. A) AAK phosphorylation at Thr243; top panel shows a representative Western blot of phosphorylated AAK (pAAK), the graph represents the densitometric analysis. B-D) Relative expression of aak-2 and aak-1 genes normalized to endogenous 18S rRNA gene expression. Each graph shows the mean ± SEM of three independent experiments, **p<0.01 vs. untreated group of its own strain, one-way ANOVA with Bonferroni's post hoc test using GraphPad Prism.