Figure 5.

661W cells were treated with 15 or 30 mM sodium formate for 24 h. The cell lysates were prepared and subjected to sodium dodecyl sulfate-polyacrylamide electrophoresis. The protein expression levels of JNK and p-JNK were determined by western blotting. GAPDH was used as an internal control to normalize the quantity of proteins applied to each lane. p-, phosphorylated; JNK, c-Jun N-terminal kinase; GADPH, glyceraldehyde-3-phosphate dehydrogenase.