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661W cells were treated with 15 or 30 mM sodium formate for 24 h. The cell lysates were prepared and subjected to sodium dodecyl sulfate-polyacrylamide electrophoresis. The protein expression levels of Bcl-2 and Bax were determined by western blotting. GAPDH was used as an internal control to normalize the amount of proteins applied in each lane. Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; GADPH, glyceraldehyde-3-phosphate dehydrogenase.
