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Immunohistochemical analysis. Tumor tissues from the control and Wnt5a-overexpressing BXPC-3 and PCNA-1 cells were immunostained with anti-Wnt5a or anti-Ki67 antibodies. Wnt5a was expressed in the cytoplasm and nuclei, whereas Ki67 was only expressed in the nuclei of the pancreatic cancer cells. Magnification, ×400.
