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Administration of TSA for 24–48 h promotes the proliferation of WB-F344 oval cells. The WB-F344 oval cells were treated with 0 (control), 10, 20 and 40 µg/ml TSA and analyzed following (A) 24, (B) 48, (C) 72 and (D) 96 h. Magnification, ×40; scale bar, 250 µm. Cell proliferation was assessed by staining with 5-ethynyl-2′-deoxyuridine and confirmed using confocal laser scanning microscopy. Proliferating cells are stained red. TSA, tanshinone IIA.
