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. 2015 Dec 18;13(2):1501–1508. doi: 10.3892/mmr.2015.4696

Figure 3.

Figure 3

TSA fails to induce apoptosis in WB-F344 hepatic oval cells. The WB-F344 oval cells were treated with 0 (control), 10, 20 and 40 µg/ml TSA and analyzed following (A) 24, (B) 48, (C) 72 and (D) 96 h. Magnification, ×600; scale bar, 50 µm. Apoptosis was assessed using a TUNEL assay and confocal laser scanning microscopy was used to detect the TUNEL-positive cells. Apoptotic cells show fluorescein isothiocyanate (green) and DAPI (blue) nuclear staining. TSA, tanshinone IIA; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.