Figure 5.

TSA upregulates the protein expression levels of β-catenin in WB-F344 oval cells. (A) WB-F344 oval cells were treated with 0 (control), 10, 20 and 40 µg/ml TSA and analyzed after 24–96 h. Immunofluorescence was used to assess the expression β-catenin (red). Cell nuclei were counter-stained blue with DAPI. Magnification, ×600; scale bar, 50 µm. (B) Number of β-catenin-positive stained cells in five randomly-selected fields were counted and plotted. Data are expressed as the mean standard deviation. ^*P<0.05 and ^**P<0.01, vs. control. TSA, tanshinone IIA.