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. 2015 Dec 4;13(2):1227–1233. doi: 10.3892/mmr.2015.4636

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Copyright: © Yue et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of 4-PBA on the protein expression of CHOP and GRP78 in rat skin flaps following ischemia-reperfusion. CHOP and GRP78 protein expression levels were detected in the skin flap tissue samples (A) 6 and 24 h following ischemia-reperfusion by western blotting, and (B) 24 h following ischemia-reperfusion by immunofluorescence staining. Error bars represent the mean ± standard deviation from five rats/group. ^*P<0.05, vs. the control group; ^#P<0.05, vs. the rats treated with saline (NC). Green, CHOP and GRP78 staining; blue, nuclei staining with 4′,6-diamidino-2-phenylindole. NC, negative control; 4-PBA, 4-phenylbutyrate; CHOP, CCAAT/enhancer-binding protein-homologous protein; GRP78, glucose-regulated protein 78; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.