Figure 2.

LY294002 inhibits the phosphorylation of mTOR and Akt during TGF-β₂-induced EMT. (A) Western blot analyses revealed that TGF-β₂ increased the phosphorylation of Akt and mTOR, but did not alter the total expression levels of Akt or mTOR. LY294002 inhibited the phosphorylation of Akt and mTOR. Control group, cells were incubated without TGF-β₂ for 24 h; TGF-β₂, group, cells incubated with 10 ng/ml TGF-β₂ for 24 h; LY+TGF-β₂ group, cells pretreated with 20 µM LY294002 for 1 h, prior to incubation with TGF-β₂ for 24 h. Representative blots of three independent experiments are shown. (B) Data in the densitometric analyses of western blotting are presented as the mean ± standard deviation of three independent experiments (^*P<0.05). LY294002/LY, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; Akt, protein kinase B; mTOR, mammalian target of rapamycin; TFG-β₂, transforming growth factor β₂; p-, phosphorylated.