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. 2015 Dec 24;13(2):1495–1500. doi: 10.3892/mmr.2015.4717

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Copyright: © Cheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of DG on caspase activation in MG-63 cells. (A) The cells were treated with various concentrations of DG (0, 5, 10 or 20 µM) for 24 h. Total cell lysates were prepared, resolved by SDS-PAGE, and immunoblotted with the indicated antibodies to detect the cleaved forms of caspase-8, caspase-9, caspase-3 and PARP. GAPDH served as a loading control. (B) Results of cleaved caspase-8, caspase-9, caspase-3 and PARP protein levels after being normalized to the levels of GAPDH. Data are representative of three independent experiments with similar results. ^*P<0.05 and ^**P<0.001 as compared with the control group. DG, dodecyl gallate; PARP, poly (ADPribose) polymerase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.