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Quantitative analysis of the effect of ketamine on neurosteroid biosynthesis. (A) PREG, (B) 17β-estradiol and (C) testosterone. Neurons were treated with 5 µM cholesterol for 24 h in the absence or presence of 100 µM ketamine. Data are presented as the mean ± standard deviation (n=3).**P<0.01 vs. the cholesterol-treated group. PREG; pregnenolone.
