Figure 4. EGFR inhibition reduced GM-CSF and TNFα levels in mice with established allergic asthma.
(A) Study protocol; adult wild-type FVB/N mice were exposed intranasally (i.n.) to 50 μg (protein) HDM or saline 3 times a week for 6 weeks. A group of mice were also treated with HDM for 6 weeks and received intraperitoneal (i.p.) injections of Erlotinib (100mg/kg) 6 times a week for the last 3 weeks of HDM exposure. The cytokines GM-CSF (B) and TNFα (C) were measured in bronchoalveolar lavage fluid by multiplex ELISA. * p<0.05, ** p<0.01; n=6 for each group in (B,C), which represents the total number of individual mice used in vivo.