. Author manuscript; available in PMC: 2016 Jan 29.

Published in final edited form as: J Med Chem. 2016 Jan 13;59(2):592–608. doi: 10.1021/acs.jmedchem.5b01369

Table 2. Tabulation of Cellular Assay Results.

Prep. I.D.	Internal I.D. SBiX	High S100B EC₅₀ (μM) ^a	Low S100B EC₅₀ (μM) ^a

		Mean ± S.D.	Mean ± S.D.
4a	4211	11.5 ± 2.4	25.6 ± 4.0
4b	1	16.3 ± 1.7	16.7 ± 2.4
4c	4210	245.2 ± 15.5	234.0 ± 7.9
5a	4225	32.2 ± 1.5	21.2 ± 1.5
5b	4224	56.2 ± 7.0	38.9 ± 6.8
5c	4226	52.0 ± 4.5	34.5 ± 4.6
5d	4221	21.8 ± 4.4	24.3 ± 5.6
6a	4213	612.5 ± 232.9	288.9 ± 106.0
6b	4214	203.1 ± 147.7	283.6 ± 221.1
6c	4218	44.1 ± 35.0	42.7 ± 43.2
6d	4217	24.8 ± 8.9	37.9 ± 32.9
7a	4227	7.8 ± 1.6	9.2 ± 2.1
7b	4223	20.0 ± 3.0	19.0 ± 3.7
7c	4228	264.2 ± 107.3	301.2 ± 139.2
7d	4222	5.4 ± 0.9	4.8 ± 0.9
8	4236	37.1 ± 3.6	33.3 ± 0.5
9a	4232	24.3 ± 6.3	25.4 ± 6.8
9b	4230	46.3 ± 5.6	45.8 ± 6.4
10	4235	0.4 ± 0.1	0.5 ± 0.1
11	4212	130.8 ± 18.1	146.1 ± 17.4
16a	4239	51.8 ± 6.0	41.0 ± 7.0
16b	4238	863.6 ± 103.2	500.1 ± 128.6
17	29	<1.0	<1.0

The SYBR Green cell proliferation assay was done using WM115 melanoma cells transfected with shRNA^scrambled (i.e. high S100B) or shRNA^S100B (low S100B).