(A) The nucleotide and deduced amino acid sequences of CYP6AA9 from Cx. pipiens pallens. The initial codon “ATG” and the termination codon “TGA” are represented in bold letters and underlined. The conserved domain of heme binding site (FGDGPRNCIG) is boxed. The polyadenylation signal sequence “AATAAA” and a poly (A) at the 3′-untranslated region are underlined.
(B) Amino acid alignment of the CYP6AA9 gene in Cx. pipiens pallens and four other species. Asterisks and dots respectively indicate identical and similar amino acids.
(C) Phylogenetic relationships of the CYP6AA9 in Cx. pipiens pallens and four other species. Species name and GenBank Accession No.: Cx. pipiens pallens, KP162164; Cx. quinquefasciatus, XP_001847405.1; Ae. aegypti, XP_001662604.1; An. gambiae XP_312054.2; Drosophila melanogaster, NP_523628.1.