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(A) Time course of TRAM-34 block of IK1 channels expressed in HEK293 cells (n = 3). (B) Representative whole-cell recordings with 10 µM Ca2+ in the patch pipette. Currents were evoked from HEK293 cells expressing IK1 by voltage ramp commands (0.16 mV/ms) in control bath solution (black) and after TRAM-34 application (red) (1 µM; 22– 24 ˚C). (C) Scatter plot of TRAM-34 block of IK1 current (n = 3).
