Figure 5. TRAM-34 (1 µM) had no significant effect on the excitability of CA1 pyramidal cells.
(A) Representative spike trains evoked by 1s long depolarizing current (200 pA) injections from -76 mV, recorded from pyramidal cells in control medium (left, black trace) and after incubation of TRAM-34 (right, red trace). (B) Comparison of spike rates (spikes/s) between control (black) and TRAM-34 (red) treated groups evoked by depolarizing, 1 s long current pulses (0–400 pA). Mono-exponential fits were used to compare spike rates in control medium and TRAM-34 treated groups, by using the function: f(X) = A[exp(-x/τ)]+ y0. No significant differences were found between control and TRAM-34 treated groups [control, τ: 263 (58) pA; TRAM-34, τ: 371 (65) pA, N.S, p = 0.161, t-test after Box-Cox transformation (Minitab 17)].