Figure 6. TRAM-34 did not block the IsAHP in BLA pyramidal neurons.
Whole-cell voltage clamp recording from BLA pyramidal neurons. (A) Representative current traces for IsAHP current evoked from a holding potential of −50 mV under control conditions (upper traces) and with 1 µM TRAM-34 added to the pipette solution. Insets show the effects of 10 µM noradrenaline. (B) The peak IsAHP current, measured 500 ms after the voltage step is plotted under the two conditions in control and TRAM-34 loaded neurons at the indicated times after onset of the whole-cell recording configuration (break-in). The lower panel shows the same data normalized to the amplitude 2 minutes after break-in. (C) Current clamp recordings from the neurons shown in (A), discharge evoked by a 600 ms current injection. (D) Plotted are the action potential half width and number of evoked action potentials evoked by the current injection at the indicated times in control and TRAM-34.