Figure 5. ILC3s are dynamic and mobilize after anti-CD40 treatment.
(A) Tracks of motile ILCs in the centre (left) and superficial (right) 15 μm of a representative cryptopatch. (B) Track speed average of ILCs combined from 5 independent experiments before and 4-–6 hr after anti-CD40 treatment. Mean steady-state 8.9 μm/min anti-CD40 7.5 mm/min, p=0.01. (C) Displacement vectors of ILCs moving into and out of representative cryptopatches from steady state (left), 4–6 hr anti-CD40 treated mice (middle), and 4–6 hr anti-CD40 treated mice given anti-GM-CSF 24 hr before imaging (right). (D) Quantification of displacement of motile ILCs from control, anti-CD40 treated, and anti-GM-CSF/anti-CD40 treated mice. (E) Ratio of entering to exiting ILCs from control, anti-CD40, and anti-GM-CSF/anti-CD40 treated mice. Motile ILCs are defined as cells with tracks lasting more than 5 min, within 75 µm of a cryptopatch, and displacing more than 14 µm (approximately two cell lengths). *, p<0.05 One-way ANOVA with Tukey’s post test. Data are combined (n=3–5) from at least 3 independent experiments. (F) Representative image of blood vessels (red) and ILCs (green) showing ILCs present adjacent to but outside blood vessels.