Figure 6. Phosphorylation of ubiquitin-specific protease-14 (USP14) regulates global protein degradation.
The quantitative analysis of proteome change in USP14 knockout or USP14 mutant cells were performed by tandem mass tag (TMT)-isobaric labeling followed by shotgun analysis. The heat map was plotted based on the set of 87 proteins that are down-regulated greater than or equal to 1.2-fold in H4 KO cells compared to H4 WT cells or to H4 KO cells complemented with WT USP14 (KO-WT). The log base 2 of average ratios was plotted as indicated.
Figure 6—figure supplement 1. Western blotting analysis of mTOR expression in WT cells and USP14 mutant-reconstituted cells.
The cell lysates were harvested and subjected to western blotting analysis using indicated antibodies.