Figure 3. NIK-deficient CD27- γδ T cells show reduced expression of Rorc and and Sox13.
(A) Gating strategy used for sorting of CD27+ and CD27-γδ T cells. Right plot depicts post-sort reanalysis, which routinely yielded purities >95%. (B) Analysis of the expression level of Rorc, Il23r and Tbet mRNA in sorted CD27+ and CD27- γδ T cells isolated from the spleen of the indicated genotypes. Data represent mean +/- SD. (C) Analysis of the expression level of Sox13 and Egr3 in sorted CD27+ and CD27- splenic γδ T cells isolated from the indicated genotypes. (D) Backskin thickness after Aldara treatment of heterozygous control (open triangles) and Map3k14-/- animals (closed squares). Data are representative of two independent experiments. (E) IL17-A and IL17-F expression of dermal γδ T cell on day 6 after Aldara treatment.