Table 2.
2DE-LC/MS/MS analyses identified a select number of protein species determined to be consistently detectable or not detectable following cuprizone treatment (indicated in Fig. 2)
| Spot ID | Tissue | MP or SP | UniProt ID | Protein identified | Present in cuprizone | Theoretical MW (kDa)/pI | Observed MW (kDa)/pI | Mascot protein score | Sequence coverage % | Number of unique peptides matched |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Cortex | SP | Q91XV3 | Brain acid soluble protein 1 | − | 22.07/4.50 | 22.7/4.8 | 196 | 45 % | 4 |
| 2 | Cortex | SP | Q61074 | Protein phosphatase 1G | − | 59.38/4.27 | 104.7/4.4 | 126 | 13 % | 10 |
| 3 | Cortex | MP | Q91X97 | Neurocalcin | − | 22.35/5.23 | 19.8/4.9 | 110 | 10 % | 2 |
| 4 | Cortex | MP | Q9D2G2 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial | − | 49.31/9.11 | 54.2/5.2 | 209 | 17 % | 12 |
| 5 | Cortex | MP | P45376 | Aldose reductase | − | 36.05/6.71 | 36.8/6.6 | 239 | 17 % | 6 |
| 6 | Cortex | MP | P39053 | Dynamin-1 | − | 98.14/7.61 | 122.3/5.6 | 142 | 19 % | 19 |
| 7 | Cortex | MP | Q91V92 | ATP-citrate synthase | + | 120.56/7.13 | 183.8/7.0 | 59 | 7 % | 8 |
| 8 | Cortex | MP | Q61411 | GTPase HRas | + | 21.63/5.16 | 21.1/4.8 | 102 | 29 % | 7 |
| 9 | Spleen | SP | Q8R1A8 | Ornithine carbamoyltransferase, mitochondrial | + | 39.86/8.81 | 42.4/6.9 | 136 | 25 % | 11 |
| 10 | Spleen | SP | Q61176 | Arginase-1 | + | 34.96/6.51 | 46.3/6.6 | 159 | 8 % | 4 |
| 11 | Spleen | SP | Q8C196 | Carbamoyl-phosphate synthase [ammonia], mitochondrial | + | 165.71/6.48 | 186.8/6.1 | 839 | 28 % | 51 |
| 12 | Spleen | MP | P08113 | Endoplasmin | − | 92.70/4.74 | 117.7/5.0 | 73 | 8 % | 7 |
| 13 | Spleen | MP | Q922R8 | Protein disulphide isomerase A6 | − | 48.47/5.00 | 47.5/5.3 | 311 | 27 % | 10 |
| 14 | Spleen | MP | Q8BGA9 | Mitochondrial inner membrane protein | − | 84.25/6.18 | 66.3/5.3 | 340 | 25 % | 24 |
| 15 | Spleen | MP | F6S5U2 | Protein disulphide isomerase A2 | − | 58.57/4.87 | 63.7/4.7 | 580 | 40 % | 32 |
| 16 | Spleen | MP | P99024 | Tubulin beta-5 chain | − | 50.10/4.78 | 28.2/5.3 | 243 | 20 % | 14 |
| 17 | Spleen | MP | Q99PL5 | Ribosome-binding protein 1 | − | 173.23/9.35 | 93.5/5.0 | 230 | 8 % | 13 |
| 18 | Spleen | MP | Q6ZWX6 | Eukaryotic translation initiation factor 2 subunit 1 | − | 36.37/5.02 | 34.6/5.5 | 98 | 43 % | 16 |
| 19 | Spleen | MP | F6Q404 | Protein disulphide isomerase A3 | − | 57.10/5.88 | 31.6/5.8 | 518 | 25 % | 11 |
| 20 | Spleen | MP | Q9JHI5 | DnaJ homolog subfamily C member 1 | + | 30.21/5.76 | 30.4/5.2 | 191 | 33 % | 11 |
| 21 | Spleen | MP | Q9JHI5 | Isovaleryl-CoA dehydrogenase | + | 46.70/8.53 | 38.9/6.0 | 213 | 10 % | 5 |
| 22 | Spleen | MP | P45376 | Aldose reductase | + | 36.05/6.71 | 33.6/6.6 | 145 | 20 % | 7 |
| 23 | Spleen | MP | Q3THE2 | Myosin regulatory light chain 12B | + | 19.82/4.71 | 18.6/4.7 | 193 | 29 % | 7 |
| 24 | Spleen | MP | Q02788 | Collagen alpha-2(VI) chain | + | 111.41/6.01 | 186.8/5.9 | 268 | 8 % | 10 |
| 25 | Spleen | MP | Q8C196 | Carbamoyl-phosphate synthase [ammonia], mitochondrial | + | 165.71/6.48 | 186.8/6.1 | 140 | 16 % | 23 |
| 26 | Spleen | MP | P29351 | Tyrosine-protein phosphatase non-receptor type 6 | + | 67.86/7.66 | 57.9/7.2 | 100 | 11 % | 7 |
| 27 | Spleen | MP | P08113 | Endoplasmin | + | 92.70/4.74 | 88.4/4.7 | 195 | 20 % | 20 |
| 28 | Spleen | MP | P11983 | T-complex protein 1 subunit alpha | + | 60.87/5.82 | 59.0/5.4 | 211 | 28 % | 18 |
| 29 | Spleen | MP | Q8BMS1 | Trifunctional enzyme subunit alpha, mitochondrial | + | 83.30/9.24 | 70.7/9.6 | 320 | 27 % | 22 |
| 30 | Spleen | MP | D3YUF7 | Prelamin-A/C | + | 74.48/6.54 | 61.2/5.8 | 416 | 33 % | 20 |
| 31 | Spleen | MP | Q9D154 | Leukocyte elastase inhibitor A | + | 42.72/5.85 | 38.4/5.4 | 183 | 36 % | 15 |
| 32 | PBMC | T | Q8R146 | Acylamino-acid-releasing enzyme | − | 82.44/5.36 | 109.6/4.9 | 361 | 22 % | 22 |
| 33 | PBMC | T | Q60605 | Myosin light polypeptide 6 | − | 17.10/4.56 | 19.1/4.4 | 85 | 31 % | 5 |
| 34 | PBMC | T | P08003 | Protein disulphide isomerase A4 | − | 72.30/5.16 | 94.9/4.7 | 211 | 24 % | 27 |
| 35 | PBMC | T | P26039 | Talin 1 | − | 271.82/5.84 | 290.5/5.4 | 3259 | 45 % | 142 |
| 36 | PBMC | T | Q8R5L1 | Complement component 1 Q subcomponent-binding protein, mitochondrial | − | 31.34/4.77 | 34.6/4.4 | 142 | 14 % | 4 |
All identified proteins were from Mus musculus
+ Detectable in cuprizone treatment
− Not detectable in cuprizone treatment
MP membrane protein fraction, SP soluble protein fraction, T total protein fraction, MW molecular weight