Figure 1.

T Cell Development and Homeostasis within Lck^Cre/+Map3k1^f/f Mice

(A) A schematic diagram representing the construction of the Map3k1^f/f allele.

(B) Thymocytes, splenocytes, bone marrow, and liver cells from WT, Map3k1^ΔKD, and Lck^Cre/+Map3k1^f/f mice (all on the C57BL/6 background) were isolated, stained with anti-CD4 and anti-CD8 antibodies, and analyzed by flow cytometry as indicated. Data are representative of three independent experiments. Numbers in the profiles indicate the percentages of the gated populations.

(C) The average percentage (±SEM) of three cell populations CD4⁺CD8⁺, CD4⁺CD8⁻, and CD4⁻CD8⁺ cells from Lck^Cre/+Map3k1^f/f and WT mice from six independent experiments was statistically analyzed (green circle, CD4⁺CD8⁺; purple square, CD4⁺CD8⁻; blue triangle, CD4⁻CD8⁺), where appropriate, by two-tailed Student’s t test (^∗p ≤ 0.05; ^∗∗p ≤ 0.01; ^∗∗∗p ≤ 0.001).