Figure 2.

iNKT Cell Development and Homeostasis in Map3k1^ΔKD and Lck^Cre/+Map3k1^f/f Mice

(A) Cell suspensions were isolated from the spleen, thymus, bone marrow, and liver from WT, Map3k1^ΔKD, and Lck^Cre/+Map3k1^f/f mice, stained with CD1d tetramer and anti-CD3 antibody, and analyzed by flow cytometry as indicated. Data are representative of five independent experiments. Numbers in the profiles indicate the percentages of the gated populations.

(B) Statistical analysis of iNKT populations (CD1d tetramer⁺CD3⁺) within the spleen, thymus, bone marrow, and liver from WT, Map3k1^ΔKD, and Lck^Cre/+Map3k1^f/f mice. The average percentage (±SEM) of CD1d-tetramer and CD3-positive cells from five independent experiments is shown (black circle, WT; red square, Map3k1^ΔKD; purple triangle, Lck^Cre/+Map3k1^f/f mice). Statistical differences were analyzed by two-tailed Student’s t test (^∗p ≤ 0.05; ^∗∗p ≤ 0.01; ^∗∗∗p ≤ 0.001).

(C) Statistical analysis of iNKT populations (CD1d tetramer⁺TCRβ⁺) within the spleen, thymus, bone marrow, and liver from WT, Map3k1^ΔKD, and Lck^Cre/+Map3k1^f/f mice. The average percentage (±SEM) of CD1d-tetramer and TCRβ-positive cells from five independent experiments is shown (black circle, WT; green square, Map3k1^ΔKD; green triangle, Lck^Cre/+Map3k1^f/f mice). Statistical differences were analyzed by two-tailed Student’s t test (^∗p ≤ 0.05; ^∗∗p ≤ 0.01; ^∗∗∗p ≤ 0.001).