Levels of renal Cyp24a1 mRNA and bone Fgf23 mRNA are elevated in GsαSglt2KO mice. A, Whole kidney levels of Cyp24a1, Cyp27b1, Vdr, Slc34a1, and Slc34a3 mRNA in control (n = 7–8 for Cyp24a1 and Cyp27b1; n = 4 for Vdr, Slc34a1, and Slc34a3) and GsαSglt2KO (n = 9–10 for Cyp24a1 and Cyp27b1; n = 5 for Vdr, Slc34a1, and Slc34a3) littermates. B, Bone levels of Fgf23, Pth1r, Vdr, Tnfsf11, and Tnfrsf11b mRNA in control (n = 8 for Fgf23; n = 7 for the others) and GsαSglt2KO (n = 8 for Fgf23; n = 11 for the others) mice. Measurements were done by using qRT-PCR relative to β-actin and normalized to values obtained from control littermates; *, P < .05; NS, not statistically significant; P ≤ .10 and P ≥ .05 are shown above the datasets.