Table 1.
qPCR Assays for PCRa
| Gene | IDT Assay Identification | Accession Number | Exons | Amplicon, bp | Amplicon Location, bp |
|---|---|---|---|---|---|
| Prl | Mm.PT.58.21865636 | NM_011164 | 3–4 | 142 | 266–407 |
| Ppia | Mm.PT.39a.2.gs | NM_008907 | 4–5 | 85 | 355–439 |
| Rps29 | Custom | NM_009093 | 1–2 | 127 | 119–245 |
| Ar | Mm.PT.47.17416675 | NM_013476 | 4–5 | 125 | 2065–2189 |
| Esr1 | Mm.PT.47.16003033 | NM_007956 | 6–7 | 100 | 1456–1555 |
| Esr2 | Mm.PT.47.17681375 | NM_207707 | 4–5 | 115 | 1036–1150 |
Abbreviation: IDT, Integrated DNA Technologies.
a
PrimeTime qPCR assays were from Integrated DNA Technologies (www.idtdna.com/pages/products/gene-expression/primetime-qpcr-assays-and-primers).