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. 2015 Nov 19;157(2):722–736. doi: 10.1210/en.2015-1281

Figure 1.

Figure 1.

Generation and characterization of the Col2.3-hWNT16-TG mice. The hWNT16 cDNA was cloned into pJ251 plasmid between the osteoblast-specific rat 2.3-kb α1 type 1 collagen promoter and mouse protamine 1 polyA sequences (A). Transgene expression (hWNT16) in various tissues (bone, skin, liver, spleen, heart, and muscle) was measured in both male and female WT and TG mice by real-time PCR. Higher level of hWNT16 was observed in bone tissue compared with other tissues (B). Transgene expression (hWNT16) in bone tissue was compared between WT and TG mice in both male and female. A high level of hWNT16 mRNA was detected only in the TG mice (C). The levels of mWnt16 (endogenous) mRNA expression were determined in both WT and TG male and female mice. The mWnt16 expression level was decreased almost 50% in both male and female TG mice compared with WT mice (D). The expression of hWNT16 in the distal femur (predominantly trabecular bone) was similar to the level of expression in the femur midshaft (cortical bone) in the TG mice (E); *, P < .05.