Figure 10.

Bone marrow-derived osteoblast and osteoclast gene expression analysis. Osteoblasts differentiated from bone marrow from WT and TG mice showed a trend of higher level of Alp in female and significantly higher level of OC expression in both male and female TG mice compared with their WT littermates (A and B). The ratio of Opg to Rankl expression was similar between the genotypes (C). Osteoclasts generated from bone marrow of WT and TG WNT16 mice demonstrated no difference of Dcstamp, Nfatc1, Trap, and Ctsk (D–G) between WT and TG mice in male; however, we observed a trend of lower expression of Ctsk (G) and significantly lower expression of Dcstamp (D) in female TG osteoclasts compared with WT cells; *, P < .05.