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Analyses of gene expression in the bone tissue. The mRNA levels of Alp and OC were higher in the bone tissue of both male and female TG WNT16 mice compared with the sex-matched WT littermates (A and B). In addition, expression of Opg was up-regulated in the same tissue, whereas Rankl expression did not change resulting in higher Opg to Rankl ratio in the TG male mice compared with the WT mice (C–E); *, P < .05.
