Figure 4.

Lack of RL-T1 or RL-D5 blunts the activation-induced increase in RANKL expression of CD4⁺T cells. A–F, CD4⁺ T cells were isolated by negative selection from spleens of 4-month-old female mice. Isolated CD4⁺ T cells were treated with vehicle (Veh) or activated (Act) with CD3/CD28 Dynabeads for 3 hours. A–F, IL-2 (A–C) and RANKL (D–F) mRNA levels were measured by RT-PCR. All values represent mean fold changes (±SD) relative to that of the WT vehicle-treated group (n = 4–6 wells/group). Statistical comparisons were performed using two-way ANOVA *, P < .05 effect of treatment within genotype; #, P < .05 effect of genotype within treatment.