Figure 7.

Lack of RL-D5 or RL-D6 blunts the LPS-induced increase in RANKL expression in vivo. A–J, 2- to 4-month-old female D5KO (A, D, and G), D6KO (B, E, and H), and T1KO (C, F, J) mice or their control littermates were injected with vehicle (V) or 10 mg/kg LPS. Tissues were collected 6 hours after a single injection. A–J, IL-6 (A–C) and RANKL (D–F) mRNA levels of lumbar vertebra 5 and RANKL (G–J) mRNA levels of femur shafts were measured by RT-PCR analysis. All values represent mean fold changes (±SD) relative to those for the WT vehicle-treated group (n = 4–7 animals/group). Statistical comparisons were performed using two-way ANOVA. *, P < .05 effect of treatment within genotype; #, P < .05 effect of genotype within treatment.