Figure 9.

Lack of RL-D6 does not alter the dietary calcium deficiency–induced increase in RANKL expression or bone loss. A–D, 1-month-old female (A and B) and 6-month-old male (C and D) D6KO and control mice were fed a calcium-deficient diet (ca-def diet) or control diet for 7 or 30 days, respectively. A and C, RANKL mRNA levels of lumbar vertebra 5 (L5) were measured with RT-PCR at day 7 in 1-month-old mice (A) and at day 30 in 6-month-old mice (C). B and D, BMDs were measured at the beginning and at the end of diet change and are represented here as percent change. All RT-PCR values represent mean fold changes relative to those for the corresponding WT control diet group. All statistical comparisons were performed using two-way ANOVA. *, P < .05 effect of diet within the same genotype; #, P < .05 effect of genotype within the same diet.