VMHGsKO mice have improved glucose metabolism on a HFD. A–D, Glucose tolerance tests in male (A and C) and female (B and D) VMHGsKO and control mice maintained on a HFD for 2 months (A and B) or 5 months (C and D) (n = 5–17/group). E–H, Insulin tolerance tests in male (E and G) and female (F and H) VMHGsKO and control mice maintained on a HFD for 2 months (E and F) or 5 months (G and H) (n = 6–11/group). Area under the curves for glucose tolerance tests and areas below baseline for insulin tolerance tests are shown to the right of each panel. I and J, Blood glucose levels during fasted and fed states in male (I) and female (J) VMHGsKO and control mice maintained on a HFD for 5 months (n = 8–12/group). *, P < .05; **, P < .01.