Figure 7.

G_sα is not imprinted in the VMH. A and B, Body weight (growth) curves of male mVMHGsKO (A) and pVMHGsKO (B) and their respective littermate controls maintained on regular chow diet. C, Body weight and composition of male mVMHGsKO and pVMHGsKO mice and their respective control littermates. D and E, Glucose tolerance tests performed on 12-week-old male mVMHGsKO (D) and pVMHGsKO (E) and their respective controls. Areas under curves are shown to the right of each panel. F, Representative VMH sections from control (left panel), mBrGsKO (middle panel), and pBrGsKO (right panel) showing in situ hybridization for G_sα mRNA (upper panels) and hematoxylin and eosin (H&E) staining (lower panels). Arrows and dashed outlines indicate VMH. Scale bar, 100 μm. In situ hybridization with a sense G_sα probe shows no signal (not shown). G, Quantitation of VMH G_sα mRNA expression levels based on in situ hybridization (n = 5–10/group). *, P < .01 vs Control.